Co-infection with Cucumber vein yellowing virus and Cucurbit yellow stunting disorder virus leading to synergism in cucumber

Study of resistance to cucumber mosaic virus watermelon mosaic virus and zucchini yellow mosaic virus in melon cultivars

Assessing the Movement of Cucurbit yellow stunting disorder virus in Susceptible and Tolerant Cucumber Germplasms Using Serological and Nucleic Acid-based Methods

Cucurbit yellow stunting disorder virus (CYSDV) is an emerging virus causing significant yield losses in cucurbits. Simple but reliable detection and quantification methods are important tools for disease management. In susceptible germplasm, CYSDV was detected 5 days postinoculation (dpi) by reverse transcriptase polymerase chain reaction (RT-PCR) or by tissue blot immunoassay (TBIA), and 8–9 ...

In Vitro Synthesized RNA Generated from cDNA Clones of Both Genomic Components of Cucurbit yellow stunting disorder virus Replicates in Cucumber Protoplasts

Cucurbit yellow stunting disorder virus (CYSDV), a bipartite whitefly-transmitted virus, constitutes a major threat to commercial cucurbit production worldwide. Here, construction of full-length CYSDV RNA1 and RNA2 cDNA clones allowed the in vitro synthesis of RNA transcripts able to replicate in cucumber protoplasts. CYSDV RNA1 proved competent for replication; transcription of both polarities...

An appropriate method to determine the interaction type of Cucumber mosaic virus (CMV) and Bean yellow mosaic virus (BYMV)

The occurrence of viral co-infection is a common phenomenon in cultivated and native plant species and can alter the dynamics of virus infection. In this study, disease progress was examined in single and mixed infections of Cucumber mosaic virus (CMV) and Bean yellow mosaic virus (BYMV) by measuring the rate of symptom development, disease severity and area under disease progress curve on infe...

Geographic distribution and phylogenetic analysis of cucurbit yellow stunting disorder virus in Iran.

Cucurbit yellow stunting disorder virus (CYSDV) is a destructive virus of cucurbits in Iran. During 2008-2012 growing seasons a total of 366 cucurbit samples including melon, cucumber, snakemelon and squash with typical symptoms of CYSDV infection were collected from ten Provinces in Iran. They were screened by ELISA and the infectivity of ELISA-positive samples was confirmed by RT-PCR. The res...